ERG Recombinant Rabbit Monoclonal Antibody [SP06-04] (ET1604-21)
Catalog# ET1604-21
Rabbit Monoclonal to ERG
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ERG Recombinant Rabbit Monoclonal Antibody [SP06-04] (ET1604-21)
Immunogen
Synthetic peptide within human erg aa 430-479 / 479.
Host
Rabbit
Positive Control
Jurkat cell lysates, CRC, PC-3M, human colon carcinoma tissue, mouse brain tissue, mouse heart tissue, human spleen tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP06-04
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Tissue
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1:200-1:500
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ERG
SYNONYMS
Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody;D030036I24Rik antibody;Erg 3 antibody;Erg antibody;ERG/EWS fusion gene, included antibody;ERG/FUS fusion gene, included antibody;ERG/TMPSSR2 fusion gene, included antibody;ERG_HUMAN antibody;ERG1, included antibody;ERG2, included antibody;ets related antibody;ETS-related gene antibody;KCNH2 antibody;Oncogene ERG antibody;p55 antibody;TMPRSS2/ERG fusion antibody;transcriptional regulator ERG (transforming protein ERG) antibody;Transcriptional regulator ERG antibody;Transforming protein ERG antibody;v ets avian erythroblastosis virus E26 oncogene antibody;v ets avian erythroblastosis virus E26 oncogene related antibody;v ets erythroblastosis virus E26 oncogene homolog antibody;v ets erythroblastosis virus E26 oncogene like antibody;v ets erythroblastosis virus E26 oncogene like isoform 2 antibody;v-ets erythroblastosis virus E26 oncogene antibody;v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody;V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
SEQUENCE SIMILARITIES
Belongs to the ETS family.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
ERG (Erythroblast transformation-specific [ETS]-related gene) is a proto-oncogene, a member of the ETS family of transcription factors. Genes in the ETS family regulate embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The ERG gene encodes for a nuclear protein, also called ERG, which is involved in hematopoietic and endothelial development. ERG remains constitually expressed in endothelial cells in blood and lymphatic vessels, and in bone marrow stem cells. ERG is expressed in virtually all endothelial neoplasms including haemangioendothelioma, angiosarcoma and Kaposi sarcoma. ERG is overexpressed secondary to gene rearrangement in cases of prostate adenocarcinoma (40-50%, the only type of carcinoma which may express ERG, due to a unique TMPRSS2-ERG gene fusion), gastrointestinal stromal tumour (GIST; 40%*), synovial sarcoma (50%*), meningioma (80%), epithelioid sarcoma (40%), malignant rhabdoid tumour, acute myeloid leukemia and blastic extramedullary myeloid tumor (70%), and rarely Ewing sarcoma / primitive peripheral neuroectodemal tumour (pPNET), chondrosarcoma*, osteosarcoma*, and rhabdomyosarcoma*.
CITATIONS
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Jinliang Nan,Yinuo Lin
Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. Journal of cellular physiology, 234(11), 21166–21181.
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WB
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IF
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Human coronary artery endothelial cells (HCAECs)
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Citation
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