PRODUCT CODE: ET1703-19

ERCC1 Recombinant Rabbit Monoclonal Antibody [JM10-07] (ET1703-19)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ERCC1 on different lysates using anti-ERCC1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela       <br />
Lane 2: Human lung <br />
Lane 3: 293T       <br />
Lane 4: A549
  • Western blot analysis of ERCC1 on different lysates using anti-ERCC1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela       <br />
Lane 2: Human lung <br />
Lane 3: 293T       <br />
Lane 4: A549
  • ICC staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin.
Western blot analysis of ERCC1 on different lysates using anti-ERCC1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human lung
Lane 3: 293T
Lane 4: A549

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ERCC1 Recombinant Rabbit Monoclonal Antibody [JM10-07] (ET1703-19)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, 293T, Hela, HepG2, PANC-1, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50 IHC-P

TARGET

UNIPROT #

PROTEIN NAME

ERCC1

SYNONYMS

COFS 4 antibody; COFS4 antibody; DNA excision repair protein ERCC 1 antibody; DNA excision repair protein ERCC-1 antibody; DNA excision repair protein ERCC1 antibody; ERCC 1 antibody; ERCC1 antibody; ERCC1_HUMAN antibody; Excision repair cross complementation group 1 antibody; Excision repair cross complementing 1 antibody; Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody; Excision repair protein antibody; RAD 10 antibody; RAD10 antibody; UV 20 antibody; UV20 antibody

SEQUENCE SIMILARITIES

Belongs to the ERCC1/RAD10/SWI10 family.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1-XPF endo-nuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.