Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
All lanes: Western blot analysis of ERAP1 with anti-ERAP1 antibody [JE52-36] (ET7110-29) at 1/500 dilution.
Lane 1: Wild-type 293T whole cell lysate.
Lane 2: ERAP1 knockout 293T whole cell lysate.
ET7110-29 was shown to specifically react with ERAP1 in wild-type 293T cells. No band was observed when ERAP1 knockout sample was tested. Wild-type and ERAP1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ERAP1 antibody (ET7110-29, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
A LAP antibody;A-LAP antibody;Adipocyte derived leucine aminopeptidase antibody;Adipocyte-derived leucine aminopeptidase antibody;ALAP antibody;Aminopeptidase PILS antibody;Aminopeptidase regulator of TNFR1 shedding antibody;APPILS antibody;ARTS 1 antibody;ARTS-1 antibody;Arts1 antibody;Endoplasmic reticulum aminopeptidase 1 antibody;Endoplasmic reticulum aminopeptidase associated with antigen processing antibody;ERAAP antibody;ERAAP1 antibody;ERAP 1 antibody;Erap1 antibody;ERAP1_HUMAN antibody;KIAA0525 antibody;kiaa0525 protein antibody;PILS AP antibody;PILS-AP antibody;PILSA antibody;PILSAP antibody;Puromycin insensitive leucyl specific aminopeptidase antibody;Puromycin-insensitive leucyl-specific aminopeptidase antibody;Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody;type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody;VEGF-induced aminopeptidase antibody
Belongs to the peptidase M1 family.
Endoplasmic reticulum membrane.
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. ERAP1 is also designated adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). Peptides presented by MHC class I on the surface of a cell must be eight to eleven residues long and ERAP1 specifically trims peptides of nine amino acids or more. ERAP1 is induced by interferon-g and encoded for by the ARTS1 gene, which maps to human chromosome 5q15. ERAP1 is thought to inactivate several bioactive peptides, including angioten-sin II, and subsequently, may be involved in the regulation of blood pressure. It may have a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and is characterized as a TNFR1 binding protein that promotes TNFR1 shedding.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.