ER Homeostasis Antibody Sampler Kit (HAK21038)

Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA721752) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (30 µg/Lane)
Lane 2: U-2 OS cell lysate (30 µg/Lane)
Lane 3: MCF7 cell lysate (30 µg/Lane)
Lane 4: HeLa cell lysate (30 µg/Lane)
Lane 5: HepG2 cell lysate (30 µg/Lane)
Lane 6: TT cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: NIH/3T3 cell lysate (30 µg/Lane)
Lane 9: C2C12 cell lysate (30 µg/Lane)
Lane 10: PC-12 cell lysate (30 µg/Lane)
Lane 11: C6 cell lysate (30 µg/Lane)
Lane 12: Mouse liver tissue lysate (30 µg/Lane)
Lane 13: Rat liver tissue lysate (30 µg/Lane)

Predicted band size: 55 kDa
Observed band size: 70 kDa

Exposure time: 6 minutes 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.