PRODUCT CODE: ET1705-33

Ephrin B2 Recombinant Rabbit Monoclonal Antibody [JM53-21] (ET1705-33)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution.<br />
Positive control:<br />
  Lane 1: Human kidney<br />
     Lane 2: Mouse lung <br />
    Lane 3: Rat brain
  • Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution.<br />
Positive control:<br />
  Lane 1: Human kidney<br />
     Lane 2: Mouse lung <br />
    Lane 3: Rat brain
  • ICC staining Ephrin B2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ephrin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Ephrin B2 on different tissue lysate using anti-Ephrin B2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human kidney
Lane 2: Mouse lung
Lane 3: Rat brain

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Ephrin B2 Recombinant Rabbit Monoclonal Antibody [JM53-21] (ET1705-33)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human kidney, mouse lung, rat brain, Hela, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM53-21

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ephrin B2

SYNONYMS

EFN B2 antibody; EFNB 2 antibody; Efnb2 antibody; EFNB2_HUMAN antibody; Eph related receptor tyrosine kinase ligand 5 antibody; EPH-related receptor tyrosine kinase ligand 5 antibody; ephrin B2 antibody; Ephrin-B2 antibody; EphrinB2 antibody; EPLG 5 antibody; EPLG5 antibody; Htk L antibody; HTK ligand antibody; HTK-L antibody; HTKL antibody; LERK 5 antibody; LERK-5 antibody; LERK5 antibody; Ligand of eph related kinase 5 antibody; MGC126226 antibody; MGC126227 antibody; MGC126228 antibody; OTTMUSP00000024973 antibody

SEQUENCE SIMILARITIES

Belongs to the ephrin family.

TISSUE SPECIFICITY

Lung and kidney.

POST-TRANSLATIONAL MODIFICATION

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Ephrin-B and are conserved cytoplasmic tyrosine residues that are Eph receptors and ephrins optimistic supporting expression in many cases during embryogenesis and ephrin-B proteins may occur in expression domain interfaces. The transmembrane ligand ephrin-B2 and its receptor tyrosine Order a copy of this thesis Bidirectional signals mediated by both proteins play an important role in vascular development. Ephrin-B2 is essential for the normal morphogenesis of the embryonic vasculature and is angiogenic in tumors. It has been Identified as an important target of chemotheiples treatments.