EpCAM Recombinant Rabbit Monoclonal Antibody [SU03-32] (ET1608-26)
Catalog# ET1608-26
Rabbit Monoclonal to EpCAM
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of EpCAM on different lysates with Rabbit anti-EpCAM antibody (ET1608-26) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: Human small intestine tissue lysate (30 µg/Lane)
Lane 4: Mouse small intestine tissue lysate (30 µg/Lane)
Lane 5: Rat small intestine tissue lysate (30 µg/Lane)
Lane 6: Human kidney tissue lysate (30 µg/Lane)
Lane 7: Mouse colon tissue lysate (30 µg/Lane)
Lane 8: Rat colon tissue lysate (30 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35/38kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-26) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: Human small intestine tissue lysate (30 µg/Lane)
Lane 4: Mouse small intestine tissue lysate (30 µg/Lane)
Lane 5: Rat small intestine tissue lysate (30 µg/Lane)
Lane 6: Human kidney tissue lysate (30 µg/Lane)
Lane 7: Mouse colon tissue lysate (30 µg/Lane)
Lane 8: Rat colon tissue lysate (30 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35/38kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-26) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.