Epac1 Recombinant Rabbit Monoclonal Antibody [JM63-29] (ET1705-79)
Catalog# ET1705-79
Rabbit Monoclonal to Epac1
-
WB
-
IHC-P
-
IF-Tissue
-
Human
-
Mouse
-
Rat
Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 104 kDa
Observed band size: 95 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Epac1 Recombinant Rabbit Monoclonal Antibody [JM63-29] (ET1705-79)
Immunogen
Synthetic peptide within human epac1 aa 874-923 / 923.
Host
Rabbit
Positive Control
HUVEC cell lysate, mouse kidney tissue lysate, rat brain tissue lysate, human prostate tissue, human stomach carcinoma tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM63-29
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:50-1:200
-
IF-Tissue
-
1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 104 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Epac1
SYNONYMS
bcm910 antibody;CAMP GEFI antibody;cAMP regulated guanine nucleotide exchange factor I antibody;CAMPGEFI antibody;CGEF 1 antibody;CGEF1 antibody;EPA1 antibody;Epac 1 antibody;EPAC antibody;EPAC1 antibody;Exchange factor directly activated by cAMP 1 antibody;Exchange protein directly activated by cAMP 1 antibody;MGC21410 antibody;RAP guanine nucleotide exchange factor antibody;Rap guanine nucleotide exchange factor (GEF) 3 antibody;RAP guanine nucleotide exchange factor 3 antibody;Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody;RAPGEF3 antibody
TISSUE SPECIFICITY
Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.
SUBCELLULAR LOCATION
Endomembrane system.
FUNCTION
3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus.
