PRODUCT CODE: ET7109-86

ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE48-94] (ET7109-86)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ENTPD5 on human liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-86, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of ENTPD5 on human liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-86, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of ENTPD5 on human liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-86, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE48-94] (ET7109-86)

Immunogen

Synthetic peptide within n-terminal human entpd5.

Host

Rabbit

Positive Control

Human liver tissue, rat testis tissue, human liver cancer tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE48-94

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • IP:1:50 IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ENTPD5

SYNONYMS

CD39 like 4 antibody; AI196558 antibody; AI987697 antibody; CD39 antigen like 4 antibody; CD39 antigen-like 4 antibody; CD39 like 4 antibody; CD39L4 antibody; Ectonucleoside triphosphate diphosphohydrolase 5 antibody; ENTP5_HUMAN antibody; Entpd5 antibody; ER UDPase antibody; ER-UDPase antibody; GDPase ENTPD5 antibody; Guanosine-diphosphatase ENTPD5 antibody; MGC163357 antibody; MGC163359 antibody; mNTPase antibody; NTPDase 5 antibody; Nucleoside diphosphatase antibody; PCPH antibody; Pcph proto oncogene protein antibody; Proto oncogene CPH antibody; UDPase ENTPD5 antibody; Uridine-diphosphatase ENTPD5 antibody

SEQUENCE SIMILARITIES

Belongs to the GDA1/CD39 NTPase family.

TISSUE SPECIFICITY

Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated; high-mannose type (By similarity). Glycosylation is not essential for enzymatic activity.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Secreted.

FUNCTION

Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.