eNOS Rabbit Polyclonal Antibody (R1412-3)

UNIPROT #

P29474

PROTEIN NAME

eNOS

SYNONYMS

Constitutive NOS antibody;EC NOS antibody;EC-NOS antibody;ecNOS antibody;Endothelial nitric oxidase synthase antibody;Endothelial nitric oxide synthase antibody;Endothelial nitric oxide synthase 3 antibody;Endothelial NOS antibody;eNOS antibody;Nitric oxide synthase 3 (endothelial cell) antibody;Nitric oxide synthase 3 antibody;Nitric oxide synthase 3 endothelial cell antibody;Nitric oxide synthase endothelial antibody;Nitric oxide synthase, endothelial antibody;NOS 3 antibody;NOS III antibody;NOS type III antibody;NOS3 antibody;NOS3_HUMAN antibody;NOSIII antibody

SEQUENCE SIMILARITIES

Belongs to the NOS family.

TISSUE SPECIFICITY

Platelets, placenta, liver and kidney.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

SUBCELLULAR LOCATION

Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane.

FUNCTION

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), generates NO in blood vessels and is involved with regulating vascular function. The gene coding for eNOS is located on Chromosome 7. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with "caveolae" a component of plasma membranes surrounding cells, and the membranes of Golgi bodies within cells. eNOS localisation to endothelial membranes is mediated by cotranslational N-terminal myristoylation and post-translational palmitoylation.