Emerin Recombinant Rabbit Monoclonal Antibody [PSH01-85] (HA721743)
Catalog# HA721743
Rabbit Monoclonal to Emerin
Tested for
-
WB
-
IHC-P
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IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Emerin on different lysates with Rabbit anti-Emerin antibody (HA721743) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: A549 cell lysate
Lane 6: K-562 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 35 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721743) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: A549 cell lysate
Lane 6: K-562 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 35 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721743) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.