eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89] (HA721747)
Catalog# HA721747
Rabbit Monoclonal to eIF4A2
Tested for
-
WB
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (HA721747) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: PC-3M cell lysate
Lane 4: 293T cell lysate
Lane 5: MCF7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C2C12 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: COS-1 cell lysate
Lane 10: Human brain tissue lysate
Lane 11: Mouse testis tissue lysate
Lane 12: Rat testis tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721747) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: PC-3M cell lysate
Lane 4: 293T cell lysate
Lane 5: MCF7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C2C12 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: COS-1 cell lysate
Lane 10: Human brain tissue lysate
Lane 11: Mouse testis tissue lysate
Lane 12: Rat testis tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721747) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.