eIF2A Recombinant Rabbit Monoclonal Antibody [JE58-18] (ET7111-34)
Catalog# ET7111-34
Rabbit Monoclonal to EIF2A
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Rat pancreas tissue lysate (40 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: Mouse pancreas tissue lysate (40 µg/Lane)
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-34) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
eIF2A Recombinant Rabbit Monoclonal Antibody [JE58-18] (ET7111-34)
Immunogen
Recombinant protein within human eif2a aa 435-585.
Host
Rabbit
Positive Control
A431 cell lysate, PC-12 cell lysate, Rat pancreas tissue lysate, NIH/3T3 cell lysate, Mouse pancreas tissue lysate, human lung carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE58-18
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 65 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
EIF2A
SYNONYMS
"65 kDa eukaryotic translation initiation factor 2A antibody;CDA 02 antibody;CDA02 antibody;EIF 2 antibody;EIF 2A antibody;eIF-2A antibody;EIF2 antibody;eif2a antibody;EIF2A_HUMAN antibody;Eukaryotic translation initiation factor 2A 65kDa antibody;Eukaryotic translation initiation factor 2A antibody;MST089 antibody;MSTP004 antibody;MSTP089 antibody"
SUBCELLULAR LOCATION
Cytoplasm, extracellular region or secreted.
FUNCTION
This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
CITATIONS
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Shi, W., Men, L., Pi, X., Jiang, T., Peng, D., Huo, S., Luo, P., Wang, M., Guo, J., Jiang, Y., Peng, L., Lin, L., Li, S., & Lv, J.
Shikonin suppresses colon cancer cell growth and exerts synergistic effects by regulating ADAM17 and the IL-6/STAT3 signaling pathway
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WB
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Human
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Citation
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Yaxin Zhi,et al
miR-421-Mediated Suppression of FGF13 as a Novel Mechanism Ameliorating Cardiac Hypertrophy by Inhibiting Endoplasmic Reticulum Stress
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WB
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Human
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Citation
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Mao, N., Fan, Y., Liu, W., Yang, H., Yang, Y., Li, Y., Jin, F., Li, T., Yang, X., Gao, X., Cai, W., Liu, H., Xu, H., Li, S., & Yang, F.
Oxamate Attenuates Glycolysis and ER Stress in Silicotic Mice
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WB
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Mouse
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Citation
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IL-6/gp130/STAT3 signaling contributed to the activation of the PERK arm of the unfolded protein response in response to chronic β-adrenergic stimulation.
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WB
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Mouse
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Citation
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Luo, H., Shen, Y., Liao, W., Li, Q., Wu, N., Zhong, J., Xiao, C., Gan, J., Yang, Y., Dong, E., Zhang, G., Liu, B., Yue, X., Xu, L., Liu, Y., Zhao, C., Zhong, Q., & Yang, H.
The inhibition of protein translation promotes tumor angiogenic switch
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WB
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Mouse
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Citation
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ADAR1p110 promotes Enterovirus D68 replication through its deaminase domain and inhibition of PKR pathway
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WB
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Human
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Citation
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Li, Y., Cai, W., Jin, F., Wang, X., Liu, W., Li, T., Yang, X., Liu, H., Xu, H., & Yang, F.
Thalidomide Alleviates Pulmonary Fibrosis Induced by Silica in Mice by Inhibiting ER Stress and the TLR4-NF-κB Pathway
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WB
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Mouse
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Citation
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