EGR2 Recombinant Rabbit Monoclonal Antibody [JG78-39] (ET7108-57)
Catalog# ET7108-57
Rabbit Monoclonal to EGR2
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WB
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Human
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Mouse
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Rat
Lane 1: SH-SY5Y cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Daudi cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
EGR2 Recombinant Rabbit Monoclonal Antibody [JG78-39] (ET7108-57)
Immunogen
Recombinant protein within human egr2 aa 2-96 / 476.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, HepG2 cell lysate, Daudi cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG78-39
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
EGR2
SYNONYMS
AT591 antibody;CMT1D antibody;CMT4E antibody;DKFZp686J1957 antibody;E3 SUMO-protein ligase EGR2 antibody;Early growth response 2 antibody;Early growth response protein 2 antibody;EGR-2 antibody;egr2 antibody;EGR2_HUMAN antibody;FLJ14547 antibody;KROX 20 Drosophila homolog antibody;Krox 20 homolog Drosophila antibody;KROX-20, Drosophila, homolog (early growth response-2) antibody;KROX20 antibody;Krox20 protein antibody;Zinc finger protein Krox-20 antibody
SEQUENCE SIMILARITIES
Belongs to the EGR C2H2-type zinc-finger protein family.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated by WWP2 leading to proteasomal degradation.; Acetylated at Lys-247. May be deacetylated by HDAC6, HDAC10 or SIRT1.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Binds to two specific DNA sites located in the promoter region of HOXA4. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
CITATIONS
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Xiujuan Hou;Suling Li;Ping Wang
Egr2 and 3 control inflammation,but maintain homeostasis,of PD-1high memory phenotype CD4 T cells
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FC
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CHIP
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Mouse
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Citation
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Dan Wang,et al
Targeting SphK1/S1PR3 axis ameliorates sepsis-induced multiple organ injury via orchestration of macrophage polarization and glycolysis
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WB
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mice
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Citation
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