PRODUCT CODE: ET7111-41

EGR1 Recombinant Rabbit Monoclonal Antibody [JE57-62] (ET7111-41)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of EGR1 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of EGR1 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue using anti-EGR1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-41, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of EGR1 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

EGR1 Recombinant Rabbit Monoclonal Antibody [JE57-62] (ET7111-41)

Immunogen

Recombinant fragment within n-terminal human egr1.

Host

Rabbit

Positive Control

SW480 cell lysates, human thyroid carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE57-62

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 58 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

EGR1

SYNONYMS

AT225 antibody; Early growth response 1 antibody; Early growth response protein 1 antibody; EGR 1 antibody; EGR-1 antibody; EGR1 antibody; EGR1_HUMAN antibody; G0S30 antibody; KROX 24 antibody; KROX24 antibody; Nerve growth factor-induced clone A antibody; Nerve growth factor-induced protein A antibody; NGFI-A antibody; NGFIA antibody; TIS8 antibody; Transcription factor ETR103 antibody; Transcription factor Zif268 antibody; ZIF 268 antibody; ZIF268 antibody; Zinc finger protein 225 antibody; Zinc finger protein Krox-24 antibody; ZNF225 antibody

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.