EGFR Mouse Monoclonal Antibody [A8E12] (HA601073)
Catalog# HA601073
Mouse Monoclonal to EGFR
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Monkey
Knock-Out or Knock-Down validated
☑ Relative expression (RE)
Western blot analysis of EGFR on different lysates with Mouse anti-EGFR antibody (HA601073) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: MCF7 cell lysate (low expression)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 150 kDa
Exposure time: 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601073) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of EGFR on different lysates with Mouse anti-EGFR antibody (HA601073) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: MCF7 cell lysate (low expression)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 150 kDa
Exposure time: 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601073) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.