PRODUCT CODE: M1510-3

Eftud2 Mouse Monoclonal Antibody [1-D10-A1] (M1510-3)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis on different cell lysates using anti-EFTUD2 Mouse mAb.  Positive control: <br />
  Lane 1: Jurkat <br />
  Lane 2: NIH/3T3
  • Western blot analysis on different cell lysates using anti-EFTUD2 Mouse mAb.  Positive control: <br />
  Lane 1: Jurkat <br />
  Lane 2: NIH/3T3
  • Immunocytochemical staining of SH-SY-5Y cells using anti-EFTUD2 Mouse mAb
  • Immunocytochemical staining of SW480 cells using anti-EFTUD2 Mouse mAb.
  • Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-EFTUD2 Mouse mAb.
  • Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-EFTUD2 Mouse mAb.
  • Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-EFTUD2 Mouse mAb.
  • Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-EFTUD2 Mouse mAb.
  • Flow cytometric analysis of MCF-7 cells with EFTUD2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without ncubation with primary antibody; black). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Western blot analysis on different cell lysates using anti-EFTUD2 Mouse mAb. Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Eftud2 Mouse Monoclonal Antibody [1-D10-A1] (M1510-3)

Immunogen

Peptide

Host

Mouse

Positive Control

Jurkat, NIH/3T3, SH-SY-5Y, SW480, mouse colon tissue, human lung cancer tissue, mouse prostate tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-D10-A1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

116 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Eftud2

SYNONYMS

116 kDa antibody; 116 kDa U5 small nuclear ribonucleoprotein component antibody; EFTUD2 antibody; Elongation factor Tu GTP binding domain containing 2 antibody; Elongation factor Tu GTP-binding domain-containing protein 2 antibody; hSNU114 antibody; MFDGA antibody; MFDM antibody; SNRNP116 antibody; Snrp116 antibody; Snu114 antibody; SNU114 homolog antibody; U5 116KD antibody; U5 small nuclear ribonucleoprotein component antibody; U5 snRNP specific protein, 116 kD antibody; U5 snRNP specific protein, 116 kDa antibody; U5 snRNP-specific protein antibody; U5-116 kDa antibody; U5-116KD antibody; U5S1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

Spliceosomes are multi-protein complexes that are composed of snRNPs (small nuclear ribonucleoproteins) and a variety of associated protein factors, all of which work in concert to regulate the splicing of pre-mRNA. Snrp116, also known as EFTUD2 (elongation factor Tu GTP binding domain containing 2) or Snu114, is a 972 amino acid protein that localizes to the nucleus and belongs to the GTP-binding elongation factor family. Existing as a component of the multi-protein U5 snRNP spliceosome complex, Snrp116 plays an important role in pre-mRNA splicing, as well as in the recycling of spliceosomal snRNPs. The gene encoding Snrp116 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.