Lane 1: NIH/3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate
Lane 5: A431 cell lysate
Lane 6: Rat brain tissue lysate
Rabbit polyclonal primary
EEA1 Rabbit Polyclonal Antibody (R1401-23)
Synthetic peptide of the n terminal residues of human eea1.
NIH/3T3, Hela, Jurkat, 293T, A431, rat brain tissue, rat prostate tissue, mouse colon tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified.
Early endosome antigen 1 antibody; Early endosome antigen 1, 162kD antibody; Early endosome associated protein antibody; EEA 1 antibody; EEA1 antibody; EEA1_HUMAN antibody; Endosome associated protein p162 antibody; Endosome-associated protein p162 antibody; MST105 antibody; MSTP105 antibody; ZFYVE2 antibody; Zinc finger FYVE domain containing protein 2 antibody; Zinc finger FYVE domain-containing protein 2 antibody
Cytoplasm, early endosome membrane.
EEA1 localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms a homodimer through a coiled coil . EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole. Mycobacterium tuberculosis is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII.