Recombinant Rabbit monoclonal primary
EBP50 Recombinant Rabbit Monoclonal Antibody [JJ0946] (ET1701-96)
MCF-7, rat kidney tissue, human kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
EBP 50 antibody; EBP50 antibody; Ezrin radixin moesin binding phosphoprotein 50 antibody; Ezrin-radixin-moesin-binding phosphoprotein 50 antibody; Na(+)/H(+) exchange regulatory cofactor NHE RF antibody; Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody; Na+/H+ exchange regulatory co factor antibody; NHERF 1 antibody; NHERF antibody; NHERF-1 antibody; NHERF1 antibody; NHRF1_HUMAN antibody; NPHLOP2 antibody; Regulatory cofactor of Na(+)/H(+) exchanger antibody; Slc9a3r1 antibody; Sodium hydrogen exchanger regulatory factor 1 antibody; Sodium-hydrogen exchanger regulatory factor 1 antibody; Sodium/hydrogen exchanger regulatory factor 1 antibody; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody; Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody; Solute carrier family 9 isoform 3 regulatory factor 1 antibody; Solute carrier family 9 isoform A3 regulatory factor 1 antibody; Solute carrier family 9 member 3 regulator 1 antibody
Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
Phosphorylated on serine residues.
Cytoplasm, Apical cell membrane, Endomembrane system, Cell projection.
The Na+/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation.