PRODUCT CODE: ET1701-96

EBP50 Recombinant Rabbit Monoclonal Antibody [JJ0946] (ET1701-96)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

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+
Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.
  • Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

EBP50 Recombinant Rabbit Monoclonal Antibody [JJ0946] (ET1701-96)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, rat kidney tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0946

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

EBP50

SYNONYMS

EBP 50 antibody; EBP50 antibody; Ezrin radixin moesin binding phosphoprotein 50 antibody; Ezrin-radixin-moesin-binding phosphoprotein 50 antibody; Na(+)/H(+) exchange regulatory cofactor NHE RF antibody; Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody; Na+/H+ exchange regulatory co factor antibody; NHERF 1 antibody; NHERF antibody; NHERF-1 antibody; NHERF1 antibody; NHRF1_HUMAN antibody; NPHLOP2 antibody; Regulatory cofactor of Na(+)/H(+) exchanger antibody; Slc9a3r1 antibody; Sodium hydrogen exchanger regulatory factor 1 antibody; Sodium-hydrogen exchanger regulatory factor 1 antibody; Sodium/hydrogen exchanger regulatory factor 1 antibody; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody; Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody; Solute carrier family 9 isoform 3 regulatory factor 1 antibody; Solute carrier family 9 isoform A3 regulatory factor 1 antibody; Solute carrier family 9 member 3 regulator 1 antibody

TISSUE SPECIFICITY

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine residues.

SUBCELLULAR LOCATION

Cytoplasm, Apical cell membrane, Endomembrane system, Cell projection.

FUNCTION

The Na+/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation.