PRODUCT CODE: ET7106-99

EB3 Recombinant Rabbit Monoclonal Antibody [JU38-30] (ET7106-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse brain tissue<br />
   Lane 2: K562
  • Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse brain tissue<br />
   Lane 2: K562
  • ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: K562

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

EB3 Recombinant Rabbit Monoclonal Antibody [JU38-30] (ET7106-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, PC-3M, SH-SY-5Y, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, mouse fallopian tubes tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU38-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

EB3

SYNONYMS

APC binding protein antibody; EB 3 antibody; EB1 protein family member 3 antibody; EB3 antibody; EBF 3 antibody; EBF3 antibody; EBF3 S antibody; End binding protein 3 antibody; End-binding protein 3 antibody; MAPRE 3 antibody; MAPRE3 antibody; MARE3_HUMAN antibody; Member 3 antibody; Microtubule Associated Protein antibody; Microtubule associated protein RP/EB family member 3 antibody; Microtubule-associated protein RP/EB family member 3 antibody; OTTHUMP00000200910 antibody; RP 3 antibody; RP/EB Family antibody; RP3 antibody

SEQUENCE SIMILARITIES

Belongs to the MAPRE family.

TISSUE SPECIFICITY

Predominantly expressed in brain and muscle.

SUBCELLULAR LOCATION

Cytoskeleton.

FUNCTION

EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL.