Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
EAAT1 Recombinant Rabbit Monoclonal Antibody [JA30-35] (ET1704-54)
Immunogen
Recombinant protein.
Host
Rabbit
Positive Control
Mouse brain tissue, rat brain tissue, human brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA30-35
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
ProA purified.
MOLECULAR WEIGHT
59 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
EAAT1
SYNONYMS
GLAST-1; SLC1A3; EAAT1; GLAST; GLAST1
SEQUENCE SIMILARITIES
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A3 subfamily.
TISSUE SPECIFICITY
Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
POST-TRANSLATIONAL MODIFICATION
Glycosylated.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.