Positive control:
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate
Lane 3: HepG2 cell lysate
Applications
-
WB
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
E2F2 Recombinant Rabbit Monoclonal Antibody [SN201-04] (ET1611-88)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
K562, 293T, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN201-04
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
48 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
E2F2
SYNONYMS
dE2F2 antibody; E2F transcription factor 2 antibody; E2F-2 antibody; E2F2 antibody; E2F2_HUMAN antibody; Transcription factor E2F2 antibody
SEQUENCE SIMILARITIES
Belongs to the E2F/DP family.
TISSUE SPECIFICITY
Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.