Lane 1/2: Wild-type A431 whole cell lysate (20 µg).
Lane 3/4: E-Cadherin fragment 1 knockdown A431 whole cell lysate (20 µg).
Lane 5/6: E-Cadherin fragment 2 knockdown A431 whole cell lysate (20 µg).
EM0502 was shown to specifically react with E-Cadherin in wild-type A431 cells. Weakened bands were observed when E-Cadherin knockdown samples were tested. Wild-type and E-Cadherin knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0502, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Mouse monoclonal primary
E-Cadherin Mouse Monoclonal Antibody [A0-G11-2] (EM0502)
A431 cell lysate, SW480 cell lysate, A431, human liver carcinoma tissue, human colon carcinoma tissue, Hela.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Arc 1 antibody; CADH1_HUMAN antibody; Cadherin 1 antibody; cadherin 1 type 1 E-cadherin antibody; Cadherin1 antibody; CAM 120/80 antibody; CD 324 antibody; CD324 antibody; CD324 antigen antibody; cdh1 antibody; CDHE antibody; E-Cad/CTF3 antibody; E-cadherin antibody; ECAD antibody; Epithelial cadherin antibody; epithelial calcium dependant adhesion protein antibody; LCAM antibody; Liver cell adhesion molecule antibody; UVO antibody; Uvomorulin antibody
Expressed in inner and outer pillar cells of the organ of Corti (at protein level). Non-neural epithelial tissues.
In the testis, expression is highest in fetal gonad, then decreases 5-fold in newborn. Detectable in 7-day-old but not in 21-day-old or adult.
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 (By similarity). Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm (By similarity). The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway (By similarity). Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system (By similarity). The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions (By similarity). During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-287 and Thr-511 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.; N-glycosylation at Asn-639 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location (By similarity).; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-756 (By similarity).
E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer.