PRODUCT CODE: EM0502

E-Cadherin Mouse Monoclonal Antibody [A0-G11-2] (EM0502)

  • IVD–IHC
  • Test-Size

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of E-cadherin on  different cell lysates using anti- E-cadherin  antibody at 1/1000 dilution.<br />
Positive control: <br />
  Lane 1: A431 <br />
  Lane 2: SW480
  • Western blot analysis of E-cadherin on  different cell lysates using anti- E-cadherin  antibody at 1/1000 dilution.<br />
Positive control: <br />
  Lane 1: A431 <br />
  Lane 2: SW480
  • ICC staining E-Cadherin in A431 cells (red).  Cells were fixed in paraformaldehyde, permeabilised  with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of  paraffin-embedded human liver carcinoma tissue  using E-Cadherin antibody. Counter stained with  hematoxylin.
  • Immunohistochemical analysis of  paraffin-embedded human colon carcinoma tissue  using E-Cadherin antibody. Counter stained with  hematoxylin.
  • Flow cytometric analysis of HeLa cells with  E-Cadherin antibody at 1/50 dilution (blue) compared  with an unlabelled control (cells without incubation  with primary antibody; red). Goat anti mouse IgG  (FITC) was used as the secondary antibody
Western blot analysis of E-cadherin on different cell lysates using anti- E-cadherin antibody at 1/1000 dilution.
Positive control:
Lane 1: A431
Lane 2: SW480

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

E-Cadherin Mouse Monoclonal Antibody [A0-G11-2] (EM0502)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

A431, SW480, MCF-7, Hela, human liver carcinoma tissue, human colon carcinoma tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A0-G11-2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

130 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

E-Cadherin

SYNONYMS

Arc 1 antibody; CADH1_HUMAN antibody; Cadherin 1 antibody; cadherin 1 type 1 E-cadherin antibody; Cadherin1 antibody; CAM 120/80 antibody; CD 324 antibody; CD324 antibody; CD324 antigen antibody; cdh1 antibody; CDHE antibody; E-Cad/CTF3 antibody; E-cadherin antibody; ECAD antibody; Epithelial cadherin antibody; epithelial calcium dependant adhesion protein antibody; LCAM antibody; Liver cell adhesion molecule antibody; UVO antibody; Uvomorulin antibody

TISSUE SPECIFICITY

Expressed in inner and outer pillar cells of the organ of Corti (at protein level). Non-neural epithelial tissues.

DEVELOPMENTAL STAGE

In the testis, expression is highest in fetal gonad, then decreases 5-fold in newborn. Detectable in 7-day-old but not in 21-day-old or adult.

POST-TRANSLATIONAL MODIFICATION

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 (By similarity). Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm (By similarity). The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway (By similarity). Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system (By similarity). The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions (By similarity). During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-287 and Thr-511 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.; N-glycosylation at Asn-639 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location (By similarity).; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-756 (By similarity).

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer.