PRODUCT CODE: HA500037

Dynamin 3 Rabbit Polyclonal Antibody (HA500037)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Rat

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+
Western blot analysis of Dynamin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500037, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat brain tissue lysate<br />
Lane 2: PC12 cell lysate
  • Western blot analysis of Dynamin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500037, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat brain tissue lysate<br />
Lane 2: PC12 cell lysate
  • ICC staining of Dynamin 3 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500037, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Dynamin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500037, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: PC12 cell lysate

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Dynamin 3 Rabbit Polyclonal Antibody (HA500037)

Immunogen

Synthetic peptide within human dynamin 3 aa 750-800.

Host

Rabbit

Positive Control

Rat brain tissue lysate, PC12 cell lysate, SKOV-3.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size: 97 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Dynamin 3

SYNONYMS

testicular dynamin antibody; testicular antibody; DNM 3 antibody; DNM3 antibody; DNM3 protein antibody; Dyn3 antibody; DYN3_HUMAN antibody; Dyna III antibody; DynaIII antibody; dynamin 2 antibody; Dynamin antibody; Dynamin family member antibody; Dynamin testicular antibody; Dynamin-3 antibody; Dynamin3 antibody; EC 3.6.5.5 antibody; KIAA0820 antibody; MGC70433 antibody; T dynamin antibody; T-dynamin antibody

SUBCELLULAR LOCATION

Cytoplasm, Cytoskeleton, Microtubule.

FUNCTION

Dynamin-3 is a protein that in humans is encoded by the DNM3 gene. The protein encoded by this gene is a member of the dynamin family which possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding. DNM3 is upregulated in Sézary's syndrome.Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).