PRODUCT CODE: ET1705-2

Dynamin 2 Recombinant Rabbit Monoclonal Antibody [JM61-38] (ET1705-2)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: PC-12<br />
  Lane 2: Mouse thymus<br />
  Lane 3: Hela
  • Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: PC-12<br />
  Lane 2: Mouse thymus<br />
  Lane 3: Hela
  • ICC staining Dynamin 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Dynamin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Dynamin 2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Dynamin 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: Mouse thymus
Lane 3: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Dynamin 2 Recombinant Rabbit Monoclonal Antibody [JM61-38] (ET1705-2)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, mouse thymus tissue, Hela, 293T, human kidney tissue, human fetal skeletal muscle tissue, mouse testes tissue, mouse colon tissue, mouse fallopian tube tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM61-38

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

98 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Dynamin 2

SYNONYMS

CMT2M antibody; CMTDI1 antibody; CMTDIB antibody; DI CMTB antibody; Dnm2 antibody; DYN II antibody; DYN2 antibody; DYN2_HUMAN antibody; Dynamin II antibody; Dynamin-2 antibody; Dynamin2 antibody; DynaminII antibody; DYNII antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

TISSUE SPECIFICITY

Ubiquitously expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2 (By similarity). Phosphorylated on tyrosine residues after activation of SRC (By similarity).

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Members of the dynamin family, including Dynamin I and Dynamin II, are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin I is localized to the central nervous system, while Dynamin II exhibits ubiquitous distribution with highest expression found in testis. Both dynamin proteins contain SH3 and proline-rich domains that mediate interactions between the dynamins and effectors of their GTPase activity. The interactions with these effectors, which include microtubules, acidic phospholipids and SH3 domain-containing proteins, are required for rapid endocytosis. Dynamin I appears to be recruited to clathrin coated pits by SH3 domain interaction with amphiphysin, a protein highly expressed in brain.