PRODUCT CODE: ER1802-77

Dynamin 1 Rabbit Polyclonal Antibody (ER1802-77)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Dynamin 1 on mouse brain tissue lysate using anti-Dynamin 1 antibody at 1/500 dilution.
  • Western blot analysis of Dynamin 1 on mouse brain tissue lysate using anti-Dynamin 1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Dynamin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Dynamin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Dynamin 1 antibody. Counter stained with hematoxylin.
Western blot analysis of Dynamin 1 on mouse brain tissue lysate using anti-Dynamin 1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Dynamin 1 Rabbit Polyclonal Antibody (ER1802-77)

Immunogen

Recombinant protein within human dynamin 1 aa 575-750.

Host

Rabbit

Positive Control

Mouse brain tissue, rat brain tissue, mouse cerebellum tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

97 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Dynamin 1

SYNONYMS

B dynamin antibody; D100 antibody; DNM 1 antibody; DNM antibody; DNM1 antibody; DYN1_HUMAN antibody; Dynamin antibody; Dynamin-1 antibody; Dynamin1 antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

SUBCELLULAR LOCATION

Cytoplasm. Cytoskeleton.

FUNCTION

Members of the Dynamin family, including Dynamin I and Dynamin II, are GTPase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin I is localized to the central nervous system, while Dynamin II exhibits ubiquitous distribution with highest expression found in testis. Both dynamin proteins contain SH3 and proline-rich domains that mediate interactions between the dynamins and effectors of their GTPase activity). The interactions with these effectors, which include microtubules, acidic phospholipids and SH3 domain-containing proteins, are required for rapid endocytosis. Dynamin I appears to be recruited to clathrin coated pits by SH3 domain interaction with amphiphysin, a protein highly expressed in brain.