PRODUCT CODE: 0912-1

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)

Applications

  • WB

  • IP

REACTIVITY

Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Lane 1: N-terminal FLAG-tagged recombinant protein<br />
Lane 2: C-terminal FLAG-tagged recombinant protein
Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)

Immunogen

Synthetic peptide immune sequence is cdykddddk.

Host

Rabbit

Positive Control

DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IP

  • 1:10-1:100

TARGET

PROTEIN NAME

DYKDDDDK Tag (FLAG)

SYNONYMS

DDDDK epitope tag antibody; DDDK antibody; ddk antibody; DYKDDDDK antibody; DYKDDDDK epitope tag antibody; DYKDDDDK tag antibody; ECS epitope tag antibody; ECS tag antibody; Enterokinase Cleavage Site epitope tag antibody; Enterokinase Cleavage Site tag antibody; FLAG antibody; FLAG tag antibody

FUNCTION

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

CITATIONS

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    Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.

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    DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication

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    Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins. | Frontiers in Microbiology [2020]

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    Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function

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    Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]

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    ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.

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    Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. | Infection and Immunity [2019]

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    Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation

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    Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents. | Nucleic Acids Research [2016]

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    E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice. | Biochemical Pharmacology [2018]