PRODUCT CODE: 0912-1

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)

Applications

  • WB

  • IP

REACTIVITY

Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Lane 1: N-terminal FLAG-tagged recombinant protein<br />
Lane 2: C-terminal FLAG-tagged recombinant protein
Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)

Immunogen

Synthetic peptide immune sequence is cdykddddk.

Host

Rabbit

Positive Control

DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IP

  • 1:10-1:100

TARGET

PROTEIN NAME

DYKDDDDK Tag (FLAG)

SYNONYMS

DDDDK epitope tag antibody; DDDK antibody; ddk antibody; DYKDDDDK antibody; DYKDDDDK epitope tag antibody; DYKDDDDK tag antibody; ECS epitope tag antibody; ECS tag antibody; Enterokinase Cleavage Site epitope tag antibody; Enterokinase Cleavage Site tag antibody; FLAG antibody; FLAG tag antibody

FUNCTION

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

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    TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction. | Autophagy [2019]