Menu Search
PRODUCT CODE: 0912-1

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)

Applications

  • WB

  • IP

REACTIVITY

-
+
Cart0
Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Lane 1: N-terminal FLAG-tagged recombinant protein<br /> Lane 2: C-terminal FLAG-tagged recombinant protein
Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)

Immunogen

Synthetic peptide immune sequence is cdykddddk.

Host

Rabbit

Positive Control

DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IP

  • 1:10-1:100

TARGET

PROTEIN NAME

DYKDDDDK Tag (FLAG)

SYNONYMS

DDDDK epitope tag antibody; DDDK antibody; ddk antibody; DYKDDDDK antibody; DYKDDDDK epitope tag antibody; DYKDDDDK tag antibody; ECS epitope tag antibody; ECS tag antibody; Enterokinase Cleavage Site epitope tag antibody; Enterokinase Cleavage Site tag antibody; FLAG antibody; FLAG tag antibody

FUNCTION

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

CITATIONS

  • Guo, Chuansheng et al.

    Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]

  • Wu, Y., Zhang, C., Liu, ......

    Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., Zhao, Q., Zhu, H., Liao, H., Cen, X., Xu, X., Zhang, M., Hou, T., Wang, Z., Yan, H., Yang, S., Sun, Y., Chen, Y., Wu, R., Xie, T., … Xia, H. (2021). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.

  • Huang, Bao et al.

    The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs. | Experimental & Molecular Medicine [2020]

  • Zhou, J., Li, J., Li, H.......

    Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J. (2021). The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.

  • You, Zhiyuan et al.

    Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress. | Nature Communications [2019]

  • Nucleolar protein NPM1 i......

    Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid

  • Zhao, Mengjing et al.

    E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice. | Biochemical Pharmacology [2018]

  • Hou, Mingyu et al.

    Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. | Infection and Immunity [2019]

  • Liu, Zhiyong et al.

    Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. | Nature Communications [2018]

  • Zhang, Pei-Ying et al.

    Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents. | Nucleic Acids Research [2016]

  • Zhou, Jianwei et al.

    Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins. | Frontiers in Microbiology [2020]

  • Histone Deacetylase 3 Co......

    Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation

  • Features of Nuclear Expo......

    Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus

  • You, Zhiyuan et al.

    TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction. | Autophagy [2019]

  • Caspase-8 Inhibition Pre......

    Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function