Applications
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WB
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IP
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)
Immunogen
Synthetic peptide immune sequence is cdykddddk.
Host
Rabbit
Positive Control
DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified
Isotype
IgG
APPLICATION DILUTION
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WB
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1:5,000
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IP
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1:10-1:100
TARGET
PROTEIN NAME
DYKDDDDK Tag (FLAG)
SYNONYMS
DDDDK epitope tag antibody; DDDK antibody; ddk antibody; DYKDDDDK antibody; DYKDDDDK epitope tag antibody; DYKDDDDK tag antibody; ECS epitope tag antibody; ECS tag antibody; Enterokinase Cleavage Site epitope tag antibody; Enterokinase Cleavage Site tag antibody; FLAG antibody; FLAG tag antibody
FUNCTION
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
CITATIONS
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Jinyan Gu; Jiyong Zhou
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
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WB
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CoIP
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human
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Citation
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Zhou, Jianwei et al.
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins. | Frontiers in Microbiology [2020]
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WB
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pig
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Citation
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Deng, T., Hu, B., Wang, X.,...
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
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WB
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Human
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Citation
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Liu, Zhiyong et al.
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. | Nature Communications [2018]
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WB
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mouse
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Citation
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Jihui Ping
Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus
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COIP
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human
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Citation
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Guo, Chuansheng et al.
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]
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WB
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None
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Citation
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Zhao, S., Chen, G., Kong, X...
BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection
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Wu, Y., Zhang, C., Liu, X.,...
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
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WB
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Human
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Citation
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You, Zhiyuan et al.
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress. | Nature Communications [2019]
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IP
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CO-IP
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human
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Citation
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Di Wang
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
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WB
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IP
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mice
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Citation
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Wu, X., Lai, Y., Rao, S., L...
Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.
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WB
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Nicotiana tabacum
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Citation
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Deng, T., Hu, B., Wang, X.,...
Inhibition of Antiviral Innate Immunity by Avibirnavirus VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.
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WB
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Citation
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Hou, Mingyu et al.
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. | Infection and Immunity [2019]
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WB
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Escherichia coli
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Citation
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Huang, Bao et al.
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs. | Experimental & Molecular Medicine [2020]
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WB
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IP
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human
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Citation
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Zhou, J., Qiu, Y., Zhu, N.,...
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
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IP
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Citation
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Guoqiang Xu;Xiangao Huang
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
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IP
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human
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Citation
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Zhou, J., Li, J., Li, H., Z...
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
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WB
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Citation
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Zhao, Mengjing et al.
E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice. | Biochemical Pharmacology [2018]
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WB
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mice
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Citation
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Zhang, Pei-Ying et al.
Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents. | Nucleic Acids Research [2016]
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WB
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human
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Citation
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Zhou, J., Wang, Y., Qiu, Y....
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
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WB
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Human
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Citation
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You, Zhiyuan et al.
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction. | Autophagy [2019]
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WB
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HEK293 cells
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Citation
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