DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)
Catalog# 0912-1
Rabbit Polyclonal to DYKDDDDK Tag (FLAG)
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WB
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IP
Specifications
Product Type
Rabbit polyclonal primary
Product Name
DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody (0912-1)
Immunogen
Synthetic peptide immune sequence is cdykddddk.
Host
Rabbit
Positive Control
DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Polyclonal
APPLICATION DILUTION
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WB
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1:5,000
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IP
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1:10-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
DYKDDDDK Tag (FLAG)
SYNONYMS
DDDDK epitope tag antibody;DDDK antibody;ddk antibody;DYKDDDDK antibody;DYKDDDDK epitope tag antibody;DYKDDDDK tag antibody;ECS epitope tag antibody;ECS tag antibody;Enterokinase Cleavage Site epitope tag antibody;Enterokinase Cleavage Site tag antibody;FLAG antibody;FLAG tag antibody
FUNCTION
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
CITATIONS
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Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H.
Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.
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WB
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Nicotiana tabacum
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Citation
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Zhou, Jianwei et al.
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins. | Frontiers in Microbiology [2020]
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WB
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pig
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Citation
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Hou, Mingyu et al.
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. | Infection and Immunity [2019]
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WB
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Escherichia coli
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Citation
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Wang, J., Ding, Y., Li, D., Zhu, N., Nishiyama, A., & Yuan, Y.
(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota
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WB
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Human
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Citation
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Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., Xu, Y., Zheng, X., & Zhou, J.
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
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WB
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Human
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Citation
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Zhao, L., Liu, Q., Huang, J., Lu, Y., Zhao, Y., & Ping, J.
TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
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WB
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Human
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Citation
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Deng, T., Hu, B., Wang, X., Ding, S., Lin, L., Yan, Y., Peng, X., Zheng, X., Liao, M., Jin, Y., Dong, W., Gu, J., & Zhou, J.
TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation
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WB
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Chicken
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Citation
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Chen, H., Hu, K., Xie, Y., Qi, Y., Li, W., He, Y., Fan, S., Liu, W., & Li, C.
CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation
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WB
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Citation
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Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., Zhao, Q., Zhu, H., Liao, H., Cen, X., Xu, X., Zhang, M., Hou, T., Wang, Z., Yan, H., Yang, S., Sun, Y., Chen, Y., Wu, R., Xie, T., … Xia, H.
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
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WB
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Human
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Citation
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Di Wang
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
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WB
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IP
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mice
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Citation
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Deng, T., Hu, B., Wang, X., Lin, L., Zhou, J., Xu, Y., Yan, Y., Zheng, X., & Zhou, J.
Inhibition of Antiviral Innate Immunity by Avibirnavirus VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.
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WB
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Citation
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Zhou, J., Wang, Y., Qiu, Y., Wang, Y., Yang, X., Liu, C., Shi, Y., Feng, X., Hou, L., & Liu, J.
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
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WB
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Human
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Citation
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Guoqiang Xu;Xiangao Huang
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
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IP
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human
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Citation
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You, Zhiyuan et al.
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction. | Autophagy [2019]
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WB
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HEK293 cells
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Citation
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Zhao, S., Chen, G., Kong, X., Chen, N., & Wu, X.
BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection
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Jihui Ping
Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus
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COIP
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human
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Citation
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Zhou, J., Wang, Y., Zhou, L., Qiu, Y., Zhao, J., Dai, B., Feng, X., Hou, L., & Liu, J.
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
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WB
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Human
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Citation
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Jinyan Gu; Jiyong Zhou
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
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WB
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CoIP
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human
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Citation
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Zhou, J., Qiu, Y., Zhao, J., Wang, Y., Zhu, N., Wang, D., Cui, Y., Guo, J., Sun, T., Ji, Y., Wu, Z., Zeng, P., Li, J., Feng, X., Hou, L., & Liu, J.
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins
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WB
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Pig
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Citation
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Guo, Chuansheng et al.
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]
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WB
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None
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Citation
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Zhao, Mengjing et al.
E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice. | Biochemical Pharmacology [2018]
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WB
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mice
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Citation
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Zhou, J., Qiu, Y., Zhu, N., Zhou, L., Dai, B., Feng, X., Hou, L., & Liu, J.
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
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IP
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Citation
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Huang, Bao et al.
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs. | Experimental & Molecular Medicine [2020]
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WB
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IP
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human
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Citation
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Zhang, Pei-Ying et al.
Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents. | Nucleic Acids Research [2016]
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WB
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human
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Citation
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Liu, Zhiyong et al.
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. | Nature Communications [2018]
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WB
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mouse
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Citation
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You, Zhiyuan et al.
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress. | Nature Communications [2019]
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IP
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CO-IP
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human
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Citation
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Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J.
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
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WB
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Citation
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