PRODUCT CODE: ET7110-83

DPYD Recombinant Rabbit Monoclonal Antibody [JE54-79] (ET7110-83)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of DPYD on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DPYD on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DPYD on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-83, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DPYD antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of DPYD on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DPYD Recombinant Rabbit Monoclonal Antibody [JE54-79] (ET7110-83)

Immunogen

Synthetic peptide corresponding to c-terminal human dpyd.

Host

Rabbit

Positive Control

THP-1 cell lysates, SiHa cell lysates, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE54-79

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

111 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

DPYD

SYNONYMS

DHP antibody; DHPDHase antibody; Dihydropyrimidine dehydrogenase [NADP(+)] antibody; Dihydropyrimidine dehydrogenase [NADP+] antibody; Dihydropyrimidine dehydrogenase antibody; Dihydrothymine dehydrogenase antibody; Dihydrouracil dehydrogenase antibody; DPD antibody; DPYD antibody; DPYD_HUMAN antibody; MGC132008 antibody; MGC70799 antibody; OTTHUMP00000058954 antibody

SEQUENCE SIMILARITIES

Belongs to the dihydropyrimidine dehydrogenase family.

TISSUE SPECIFICITY

Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. In humans the enzyme is encoded by the DPYD gene. It is the initial and rate-limiting step in pyrimidine catabolism.[citation needed] It catalyzes the reduction of uracil and thymine. It is also involved in the degradation of the chemotherapeutic drugs 5-fluorouracil and tegafur. It also participates in beta-alanine metabolism and pantothenate and coa biosynthesis. The protein is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Genetic deficiency of this enzyme results in an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy.