PRODUCT CODE: R1312-4

DPY30 Rabbit Polyclonal Antibody (R1312-4)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

ICC staining DPY30 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
  • ICC staining DPY30 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
  • ICC staining DPY30 in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- DPY30 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DPY30 rabbit polyclonal antibody.
ICC staining DPY30 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DPY30 Rabbit Polyclonal Antibody (R1312-4)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

D3, F9, human lung carcinoma tissue, mouse brain

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:100-1:200

  • ICC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

DPY30

SYNONYMS

Dpy-30-like protein antibody; Dpy-30L antibody; DPY30 antibody; DPY30_HUMAN antibody; HDPY-30 antibody; Protein dpy-30 homolog antibody; Saf19 antibody

SEQUENCE SIMILARITIES

Belongs to the dpy-30 family.

SUBCELLULAR LOCATION

Nucleus. Golgi apparatus

FUNCTION

As part of the MLL1/MLL complex, DPY30 is involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. DPY30 may play some role in histone H3 acetylation. In a teratocarcinoma cell, it plays a crucial role in retinoic acid-induced differentiation along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci. DPY30 may also play an indirect or direct role in endosomal transport. In the recent study, it showed that depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage.