PRODUCT CODE: M0731-6

DPPA2 Mouse Monoclonal Antibody [D0-G11] (M0731-6)

Applications

  • WB

REACTIVITY

  • Mouse

Western blot analysis of DPPA2 on F9 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0731-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of DPPA2 on F9 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0731-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of DPPA2 on F9 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0731-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

DPPA2 Mouse Monoclonal Antibody [D0-G11] (M0731-6)

Immunogen

Peptide

Host

Mouse

Positive Control

F9 cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

D0-G11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

37kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

DPPA2

SYNONYMS

cancer/testis antigen 100 antibody; CT100 antibody; Developmental Pluripotency Associated 2 antibody; developmental pluripotency-associated gene 2 antibody; developmental pluripotency-associated protein 2 antibody; ECAT15-2 antibody; embryonic stem cell (ESC) associated transcript 15-2 antibody; PESCRG1 antibody; Pluripotent embryonic stem cell related protein 1 antibody; Pluripotent embryonic stem cell-related gene 1 antibody; Pluripotent embryonic stem cell-related gene 1 protein antibody

TISSUE SPECIFICITY

Not detected in adult tissues.

DEVELOPMENTAL STAGE

Expressed in 4-cell embryo, morula and blastocyst. At 7.5 dpc, detected in the epiblast. At 10.0 dpc, expressed in the primordial germ cells. Expression decreases at 12.0 dpc in the embryonic gonads. At 14.5 dpc, expression is restricted to testis. 6 days after birth, still detected in primitive type A spermatogonia. Expressed in undifferentiated embryonic stem cells, but expression rapidly decreases upon differentiation.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

DPPA2 (developmental pluripotency associated 2) is an embryo-cancer antigen that is coexpressed with cancer-testis antigens in non-small cell lung cancer. Dppa2 is closely linked SAP motif genes restricted to pluripotent cells and the germ line. It may play a role in maintaining cell pluripotentiality and in embryo preimplantation development.