PRODUCT CODE: ET7110-75

DOK1 Recombinant Rabbit Monoclonal Antibody [JE54-52] (ET7110-75)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Rat

-
+
Western blot analysis of DOK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: K562 cell lysate<br />
Lane 2: Rat skin tissue lysate
  • Western blot analysis of DOK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: K562 cell lysate<br />
Lane 2: Rat skin tissue lysate
Western blot analysis of DOK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Rat skin tissue lysate

Applications

  • WB

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DOK1 Recombinant Rabbit Monoclonal Antibody [JE54-52] (ET7110-75)

Immunogen

Synthetic peptide corresponding to human dok1.

Host

Rabbit

Positive Control

K562 cell lysate, rat skin tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE54-52

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

DOK1

SYNONYMS

Docking protein 1 62kD antibody; Docking protein 1 antibody; DOK 1 antibody; DOK1 antibody; DOK1_HUMAN antibody; Downstream of tyrosine kinase 1 antibody; p62(dok) antibody; P62DOK antibody; pp62 antibody

SEQUENCE SIMILARITIES

Belongs to the DOK family. Type A subfamily.

TISSUE SPECIFICITY

Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.

POST-TRANSLATIONAL MODIFICATION

Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.

SUBCELLULAR LOCATION

Nucleus, cytoplasm; Perinuclear region.

FUNCTION

Docking protein 1 is a protein that in humans is encoded by the DOK1 gene. The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.