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PRODUCT CODE: ER1802-17

DOCK4 Rabbit Polyclonal Antibody (ER1802-17)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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ICC staining DOCK4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DOCK4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DOCK4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DOCK4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.
ICC staining DOCK4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DOCK4 Rabbit Polyclonal Antibody (ER1802-17)

Immunogen

Synthetic peptide within human dock4 aa 1,917-1,966 / 1,966.

Host

Rabbit

Positive Control

HepG2, HUVEC, PC-3M, rat skeletal muscle tissue, human fetal skeletal muscle tissue, human prostate tissue, mouse skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

225 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • WB

  • 1:500

TARGET

UNIPROT #

Q8N1I0

PROTEIN NAME

DOCK4

SYNONYMS

Dedicator of cytokinesis protein 4 antibody; Dock4 antibody; DOCK4_HUMAN antibody; KIAA0716 antibody

SEQUENCE SIMILARITIES

Belongs to the DOCK family.

TISSUE SPECIFICITY

Widely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary.; [Isoform 2]: May be specifically expressed in the brain and eye.

SUBCELLULAR LOCATION

Cytosol. Cell membrane.

FUNCTION

DOCK 4 (Dedicator of cytokinesis protein 4) is a cytoplasmic peripheral membrane protein that belongs to the DOCK family of cytokinesis-regulating proteins. Expressed ubiquitously with highest expression in prostate, ovary and skeletal muscle, DOCK 4 functions as a guanine nucleotide exchange factor (GEF) that activates the small GTPase Rap 1 and, via this activation, plays a role in the regulation of adherens junctions between cells. Similar to other DOCK family members, DOCK 4 contains an N-terminal SH3 domain, a C-terminal proline-rich region and two internal DOCK homology regions designated DHR1 and DHR2. Defects in the gene encoding DOCK 4 result in the inactivation of Rap 1 and are, thus, implicated in the pathogenesis of various cancers such as ovarian, prostate, glioma and colorectal carcinomas. Four isoforms of DOCK 4 are expressed due to alternative splicing events.