PRODUCT CODE: ET1605-9

Dnmt3b Recombinant Rabbit Monoclonal Antibody [SY09-07] (ET1605-9)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of Dnmt3b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: Hela cell lysate<br />
Lane 3: A431 cell lysate
  • Western blot analysis of Dnmt3b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: Hela cell lysate<br />
Lane 3: A431 cell lysate
Western blot analysis of Dnmt3b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: A431 cell lysate

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Dnmt3b Recombinant Rabbit Monoclonal Antibody [SY09-07] (ET1605-9)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549 cell lysate, Hela cell lysate, A431 cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY09-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

96 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Dnmt3b

SYNONYMS

Cytosine 5methyltransferase 3B antibody; DNA antibody; DNA (cytosine 5) methyltransferase 3 beta antibody; DNA (cytosine 5)-methyltransferase 3B antibody; DNA (cytosine-5)-methyltransferase 3B antibody; DNA methyltransferase HsaIIIB antibody; DNA MTase HsaIIIB antibody; DNM3B_HUMAN antibody; Dnmt3b antibody; EC 2.1.1.37 antibody; ICF antibody; ICF1 antibody; M.HsaIIIB antibody; MGC124407 antibody; RP23-89H14.3 antibody

SEQUENCE SIMILARITIES

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

TISSUE SPECIFICITY

Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

POST-TRANSLATIONAL MODIFICATION

Sumoylated.; Citrullinated by PADI4.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

CITATIONS

  • Xie, Binbin et al.

    Tumor-suppressive function and mechanism of HOXB13 in right-sided colon cancer. | Signal Transduction and Targeted Therapy [2019]