Dnmt1 Recombinant Rabbit Monoclonal Antibody [JF09-89] (ET1702-77)
Catalog# ET1702-77
Rabbit Monoclonal to Dnmt1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: F9 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Dnmt1 Recombinant Rabbit Monoclonal Antibody [JF09-89] (ET1702-77)
Immunogen
Synthetic peptide within human dnmt1 aa 1,506-1,549 / 1,616.
Host
Rabbit
Positive Control
HEK-293 cell lysate, HepG2 cell lysate, F9 cell lysate, C6 cell lysate, Hela, 293T, human lymph nodes tissue, HepG2, F9, human stomach tissue, mouse stomach tissue, rat stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF09-89
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:1.000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 183 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Dnmt1
SYNONYMS
ADCADN antibody;AIM antibody;CXXC finger protein 9 antibody;CXXC-type zinc finger protein 9 antibody;CXXC9 antibody;DNA (cytosine 5 ) methyltransferase 1 antibody;DNA (cytosine-5)-methyltransferase 1 antibody;DNA methyltransferase 1 antibody;DNA methyltransferase HsaI antibody;DNA methyltransferase M.HsaI. antibody;DNA MTase antibody;DNA MTase HsaI antibody;DNMT 1 antibody;DNMT antibody;Dnmt1 antibody;DNMT1_HUMAN antibody;Dnmt1o antibody;FLJ16293 antibody;HSN1E antibody;M.HsaI antibody;MCMT antibody;Met1 antibody;MGC104992 antibody;mMmul antibody;MommeD2 antibody
SEQUENCE SIMILARITIES
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
TISSUE SPECIFICITY
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
POST-TRANSLATIONAL MODIFICATION
Sumoylated; sumoylation increases activity.; Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.; Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.; Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.; Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
CITATIONS
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Punicalagin protects against impaired skeletal muscle function in high-fat-diet-induced obese mice by regulating TET2
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WB
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Mouse
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Citation
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Zhang, X., Zhao, S., Yuan, Q., Zhu, L., Li, F., Wang, H., Kong, D., & Hao, J.
TXNIP, a novel key factor to cause Schwann cell dysfunction in diabetic peripheral neuropathy, under the regulation of PI3K/Akt pathway inhibition-induced DNMT1 and DNMT3a overexpression. Cell death & disease, 12(7), 642.
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WB
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IHC
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IF
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Citation
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DNMT1, a Novel Regulator Mediating mTORC1/mTORC2 Pathway-Induced NGF Expression in Schwann Cells.
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WB
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ICC
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Rat
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Citation
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Duan, X., Huang, Y., Chen, X., Wang, W., Chen, J., Li, J., Yang, W., Li, J., Wu, Q., & Wong, J.
Moderate DNA hypomethylation suppresses intestinal tumorigenesis by promoting caspase-3 expression and apoptosis. Oncogenesis, 10(5), 38.
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WB
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Mouse
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Citation
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