DNMT1 Mouse Monoclonal Antibody [A3A8] (EM1901-83)
Catalog# EM1901-83
Mouse Monoclonal to DNMT1
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-83) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
DNMT1 Mouse Monoclonal Antibody [A3A8] (EM1901-83)
Immunogen
Synthetic peptide within human dnmt1 aa 500-600 / 1616.
Host
Mouse
Positive Control
HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse ovary tissue lysate, mouse testis tissue lysate, rat ovary tissue lysate, rat testis tissue lysate, HeLa, human testis tissue, human tonsil tissue, mouse ovary tissue, mouse testis tissue, rat ovary tissue, rat testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3A8
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:4,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:400
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 183 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
DNMT1
SYNONYMS
ADCADN antibody;AIM antibody;CXXC finger protein 9 antibody;CXXC-type zinc finger protein 9 antibody;CXXC9 antibody;DNA (cytosine 5 ) methyltransferase 1 antibody;DNA (cytosine-5)-methyltransferase 1 antibody;DNA methyltransferase 1 antibody;DNA methyltransferase HsaI antibody;DNA methyltransferase M.HsaI. antibody;DNA MTase antibody;DNA MTase HsaI antibody;DNMT 1 antibody;DNMT antibody;Dnmt1 antibody;DNMT1_HUMAN antibody;Dnmt1o antibody;FLJ16293 antibody;HSN1E antibody;M.HsaI antibody;MCMT antibody;Met1 antibody;MGC104992 antibody;mMmul antibody;MommeD2 antibody
SEQUENCE SIMILARITIES
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
TISSUE SPECIFICITY
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
POST-TRANSLATIONAL MODIFICATION
Sumoylated; sumoylation increases activity.; Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.; Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.; Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.; Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.
CITATIONS
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Yangfan Liu , Yu Sun , Jin Yang ,et al
DNMT1-targeting remodeling global DNA hypomethylation for enhanced tumor suppression and circumvented toxicity in oral squamous cell carcinoma
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WB
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IHC
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Human
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Citation
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