DNAJC6 Recombinant Rabbit Monoclonal Antibody [PSH07-95] (HA722929)
Catalog# HA722929
Rabbit Monoclonal to DNAJC6
Tested for
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of DNAJC6 on different lysates with Rabbit anti-DNAJC6 antibody (HA722929) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Human liver tissue lysate (negative)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse cerebellum tissue lysate
Lane 5: Mouse liver tissue lysate (negative)
Lane 6: Rat brain tissue lysate
Lane 7: Rat liver tissue lysate (negative)
Lane 8: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 110 kDa
Exposure time: Lane 1-8 (left): 30 seconds; Lane 1-8 (right): 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722929) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of DNAJC6 on different lysates with Rabbit anti-DNAJC6 antibody (HA722929) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Human liver tissue lysate (negative)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse cerebellum tissue lysate
Lane 5: Mouse liver tissue lysate (negative)
Lane 6: Rat brain tissue lysate
Lane 7: Rat liver tissue lysate (negative)
Lane 8: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 110 kDa
Exposure time: Lane 1-8 (left): 30 seconds; Lane 1-8 (right): 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722929) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.