PRODUCT CODE: M1204-6

DNA-PKcs/PRKDC Mouse Monoclonal Antibody [2-B3] (M1204-6)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis on HepG2 cell lysates using anti-DNA-PKcs Mouse mAb (Cat. # M1204-6).
  • Western blot analysis on HepG2 cell lysates using anti-DNA-PKcs Mouse mAb (Cat. # M1204-6).
  • Immunofluorescent staining of HepG2 cells using anti-DNA-PKcs Mouse mAb (Cat. # M1204-6).
  • Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-DNA-PKcs Mouse mAb (Cat.# M1204-6)
Western blot analysis on HepG2 cell lysates using anti-DNA-PKcs Mouse mAb (Cat. # M1204-6).

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

DNA-PKcs/PRKDC Mouse Monoclonal Antibody [2-B3] (M1204-6)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

HepG2 Hela

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-B3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

ٵ_469

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:100

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

DNA-PKcs/PRKDC

SYNONYMS

DNA dependent protein kinase catalytic subunit antibody; DNA PK catalytic subunit antibody; DNA-dependent protein kinase catalytic subunit antibody; DNA-PK catalytic subunit antibody; DNA-PKcs antibody; DNAPK antibody; DNAPK catalytic subunit antibody; DNPK 1 antibody; DNPK1 antibody; Hyper radiosensitivity of murine scid mutation, complementing 1 antibody; Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody; HYRC 1 antibody; HYRC antibody; HYRC1 antibody; IMD26 antibody; p350 antibody; p460 antibody; PKRDC antibody; PRKDC antibody; PRKDC_HUMAN antibody; Protein Kinase DNA Activated Catalytic Polypeptide antibody; XRCC 7 antibody; XRCC7 antibody

SEQUENCE SIMILARITIES

Belongs to the PI3/PI4-kinase family.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated at two clusters, the T2609 cluster and the S2056 cluster (By similarity). Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. Autophosphorylation of the T2609 cluster is required for hematopoietic development and protein synthesis in erythrocytes precursors (By similarity).; S-nitrosylated by GAPDH.; Polyubiquitinated by RNF144A, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

DNA-activated serine/threonine protein kinase (DNA-PK) is a heterotrimer of PRKDC and the Ku p70-p86 (XRCC6-XRCC5) dimer. DNA-PK is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required for double- strand brake repair and V(D)J recombination.