PRODUCT CODE: ET7107-14

DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] (ET7107-14)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

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+
Western blot analysis of DNA Ligase I on different cell lysates using anti-DNA Ligase I antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Daudi  <br />
   Lane 2: A431<br />
 Lane 3: MCF-7
  • Western blot analysis of DNA Ligase I on different cell lysates using anti-DNA Ligase I antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Daudi  <br />
   Lane 2: A431<br />
 Lane 3: MCF-7
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.
Western blot analysis of DNA Ligase I on different cell lysates using anti-DNA Ligase I antibody at 1/500 dilution.
Positive control:
Lane 1: Daudi
Lane 2: A431
Lane 3: MCF-7

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39] (ET7107-14)

Immunogen

Recombinant protein within c terminal human dna ligase.

Host

Rabbit

Positive Control

Daudi, A431, MCF-7, Rat testis tissue, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB43-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

Predicted band size 102 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

DNA Ligase I

SYNONYMS

DNA ligase 1 antibody; DNA ligase I antibody; DNLI1_HUMAN antibody; LIG 1 antibody; lig1 antibody; Ligase I DNA ATP dependent antibody; MGC117397 antibody; MGC130025 antibody; Polydeoxyribonucleotide synthase [ATP] 1 antibody

SEQUENCE SIMILARITIES

Belongs to the ATP-dependent DNA ligase family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.