DLK-1 Recombinant Rabbit Monoclonal Antibody [PSH04-76] (HA722180)
Catalog# HA722180
Rabbit Monoclonal to DLK-1
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: Mouse placenta tissue lysate (70℃ heat) (40 µg/Lane)
Lane 5: Rat embryo tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 40-50kDa
Exposure time: Lane 1-4: 2 minutes; Lane 5: 27 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
DLK-1 Recombinant Rabbit Monoclonal Antibody [PSH04-76] (HA722180)
Immunogen
Recombinant protein within human dlk-1 aa 1-327 / 383.
Host
Rabbit
Positive Control
MCF7 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, mouse placenta tissue lysate, rat embryo tissue lysate, human pancreas tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-76
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
DLK-1
SYNONYMS
Adipocyte differentiation inhibitor protein antibody;Brevideltin antibody;Delta like 1 homolog antibody;Delta like homolog antibody;Delta like protein antibody;Delta1 antibody;DLK 1 antibody;DLK antibody;DLK-1 antibody;DLK1 antibody;DLK1_HUMAN antibody;FA 1 antibody;FA1 antibody;Fetal antigen 1 antibody;pG2 antibody;Preadipocyte factor 1 antibody;Pref 1 antibody;Pref antibody;Pref1 antibody;Protein delta homolog 1 antibody;Secredeltin antibody;ZOG antibody
SUBCELLULAR LOCATION
Membrane, Cytoplasm.
FUNCTION
Protein delta homolog 1, delta like non-canonical Notch ligand 1, fetal antigen 1 or preadipocyte factor 1 is a protein that in humans is encoded by the DLK1 gene. It is expressed as a transmembrane protein, but a soluble form cleaved off by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes. It is a member of the EGF-like family of homeotic proteins. Part of the Dlk1-DIO3 imprinting control region, this gene is one involved in the epigenetic process that causes a subset of genes to be regulated based on their parental origin. Such imprinted genes are required for the formation of the placenta as well as the development of cellular lineages such as those derived from the mesoderm and ectoderm.
CITATIONS
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Zhiduan Chi,et al
snRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle
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IF
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IHC
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Mongolian cattle
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Citation
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