Dkk3 Recombinant Rabbit Monoclonal Antibody [JE35-98] (HA722396)
Catalog# HA722396
Rabbit Monoclonal to Dkk3
Tested for
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WB
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IF-Cell
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IHC-P
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IHC-Fr
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IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Dkk3 on different lysates with Rabbit anti-Dkk3 antibody (HA722396) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (14 µg/Lane)
Lane 2: Mouse brain tissue lysate treated with deglycosylation (14 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 50-70 kDa
Exposure time: Lane 1-3: 10 seconds; Lane 4: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722396) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (14 µg/Lane)
Lane 2: Mouse brain tissue lysate treated with deglycosylation (14 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 50-70 kDa
Exposure time: Lane 1-3: 10 seconds; Lane 4: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722396) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.