PRODUCT CODE: ER1512-20

DKK1 Rabbit Polyclonal Antibody (ER1512-20)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse brain  <br />
  Lane 2: Human fetal brain
  • Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse brain  <br />
  Lane 2: Human fetal brain
  • ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with DKK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: Human fetal brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DKK1 Rabbit Polyclonal Antibody (ER1512-20)

Immunogen

Synthetic peptide within c-terminal human dkk1.

Host

Rabbit

Positive Control

Mouse brain tissue lysate, human fetal brain tissue lysate, Hela, NCCIT.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DKK1

SYNONYMS

Dickkopf 1 antibody; Dickkopf 1 homolog antibody; Dickkopf 1 like antibody; Dickkopf homolog 1 antibody; Dickkopf like protein 1 antibody; Dickkopf related protein 1 antibody; Dickkopf WNT signaling pathway inhibitor 1 antibody; Dickkopf-1 antibody; Dickkopf-related protein 1 antibody; DKK 1 antibody; Dkk-1 antibody; Dkk1 antibody; DKK1_HUMAN antibody; hDkk 1 antibody; hDkk-1 antibody; SK antibody

SEQUENCE SIMILARITIES

Belongs to the dickkopf family.

TISSUE SPECIFICITY

Placenta.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3.