Dicer Rabbit Polyclonal Antibody (HA500522)
Catalog# HA500522
Rabbit Polyclonal to Dicer
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (HA500522) at 1/1,000 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 219 kDa
Observed band size: 250 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500522) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 219 kDa
Observed band size: 250 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500522) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.