PRODUCT CODE: ET1609-32

DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79] (ET1609-32)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela                 <br />
Lane 2: PC12 <br />
Lane 3: NIH/3T3
  • Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela                 <br />
Lane 2: PC12 <br />
Lane 3: NIH/3T3
  • ICC staining DGCR8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DGCR8 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC12
Lane 3: NIH/3T3

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79] (ET1609-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC12, NIH/3T3, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST04-79

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

DGCR8

SYNONYMS

DGCRK6 antibody; C22orf12 antibody; D16H22S788E antibody; D16Wis2 antibody; DGCR 8 antibody; Dgcr8 antibody; DGCR8 microprocessor complex subunit antibody; DGCR8_HUMAN antibody; DGCRK 6 antibody; DiGeorge syndrome critical region 8 antibody; DiGeorge syndrome critical region gene 8 antibody; Gy1 antibody; Microprocessor complex subunit DGCR8 antibody; pasha antibody

TISSUE SPECIFICITY

Ubiquitously expressed.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

DGS8, also designated DiGeorge syndrome critical region 8 protein, plays a role in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS). It is a ubiquitously expressed protein encoded by the gene DGCR8, which is deleted in DiGeorge syndrome. DiGeorge syndrome is characterized by structural and functional palate anomalies, conotruncal cardiac malformations, immunodeficiency, hypocalcemia, and typical facial anomalies. In mouse, DGS8 is detected primarily in embryonic brain, vessels, thymus and palate.