DFNA5/GSDME Rabbit Polyclonal Antibody (ER1901-12)
Catalog# ER1901-12
Rabbit Polyclonal to DFNA5/GSDME
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WB
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IHC-P
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FC
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Human
Lane 1: Hela cell lysate
Lane 2: Siha cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-12) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
DFNA5/GSDME Rabbit Polyclonal Antibody (ER1901-12)
Immunogen
Recombinant protein within human dfna5 / gsdme aa 1-220 / 496.
Host
Rabbit
Positive Control
Hela cell lysates, SiHa cell lysates, rat testis tissue, human thyroid gland tissue, human breast tissue, mouse small intestine tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB:1:500-1:1,000
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IHC-P:1:50-1:200
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FC:1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
DFNA5/GSDME
SYNONYMS
2310037D07Rik antibody;4932441K13Rik antibody;Deafness, autosomal dominant 5 antibody;Deafness, autosomal dominant 5 protein antibody;DFNA5 antibody;DFNA5 gene antibody;DFNA5_HUMAN antibody;Dfna5h antibody;EG14210 antibody;Fin15 antibody;ICERE 1 antibody;ICERE-1 antibody;Inversely correlated with estrogen receptor expression 1 antibody;Non-syndromic hearing impairment protein 5 antibody;Nonsyndromic hearing impairment protein antibody
SEQUENCE SIMILARITIES
Belongs to the gasdermin family.
TISSUE SPECIFICITY
Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
POST-TRANSLATIONAL MODIFICATION
Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) relieves autoinhibition and is sufficient to initiate pyroptosis.
SUBCELLULAR LOCATION
Cell membrane, Cytoplasm, cytosol.
FUNCTION
Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53. Gasdermin-E, N-terminal: Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity.
CITATIONS
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Wang Jiabin,et al
Comprehensive multi-omics analysis of pyroptosis for optimizing neoadjuvant immunotherapy in patients with gastric cancer
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IHC
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Human
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Citation
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