DENN Recombinant Rabbit Monoclonal Antibody [JE63-62] (HA721022)
Catalog# HA721022
Rabbit Monoclonal to DENN
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DENN on different lysates with Rabbit anti-DENN antibody (HA721022) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (negative) (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 183 kDa
Observed band size: 250 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (negative) (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 183 kDa
Observed band size: 250 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.