PRODUCT CODE: ET1611-92

Delta Opioid Receptor Recombinant Rabbit Monoclonal Antibody [SN20-04] (ET1611-92)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Delta Opioid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HUVEC cell lysate<br />
Lane 2: rat brain tissue lysate
  • Western blot analysis of Delta Opioid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HUVEC cell lysate<br />
Lane 2: rat brain tissue lysate
  • Flow cytometric analysis of Delta Opioid Receptor was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-92, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Delta Opioid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HUVEC cell lysate
Lane 2: rat brain tissue lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Delta Opioid Receptor Recombinant Rabbit Monoclonal Antibody [SN20-04] (ET1611-92)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC cell lysate, rat brain tissue lysate, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN20-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Delta Opioid Receptor

SYNONYMS

D-OR-1 antibody; Delta type opioid receptor antibody; Delta type opioid receptor DOR 1 antibody; Delta type opioid receptor DOR1 antibody; Delta-type opioid receptor antibody; DOR 1 antibody; DOR-1 antibody; DOR1 antibody; mDOR antibody; Nbor antibody; Opioid receptor delta 1 antibody; OPRD 1 antibody; OPRD antibody; OPRD_HUMAN antibody; Oprd1 antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Endogenous opioid peptides and opiates, like morphine, transmit their pharmacological effects through membrane bound opioid receptors. Pharmacological studies and molecular cloning have led to the identification of three different types of opioid receptor, mu-type, delta-type and kappa-type, also designated MOR-1, DOR-1 and KOR-1, respectively. MOR-1 is a receptor for beta-endorphin, DOR-1 is a receptor for enkephalins, and KOR-1 is a receptor for dynorphins. The three opioid receptor types are highly homologous and belong to the superfamily of G protein-coupled receptors. Opioid receptors have been shown to modulate a range of brain functions, including instinctive behavior and emotions. This regulation is thought to involve the inhibition of neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance.