DEK Recombinant Rabbit Monoclonal Antibody [JB36-32] (ET7107-99)
Catalog# ET7107-99
Rabbit Monoclonal to DEK
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WB
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IF-Cell
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FC
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Human
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Rat
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 50 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-99) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
DEK Recombinant Rabbit Monoclonal Antibody [JB36-32] (ET7107-99)
Immunogen
Recombinant protein within human dek aa 31-220 / 375.
Host
Rabbit
Positive Control
HeLa cell lysate, A431 cell lysate, K-562 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, A431.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB36-32
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:250
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
DEK
SYNONYMS
D6S231E antibody;Dek antibody;DEK gene antibody;DEK oncogene antibody;DEK oncogene DNA binding antibody;DEK_HUMAN antibody;Protein DEK antibody
TISSUE SPECIFICITY
Ubiquitous. Expressed at relatively high levels.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The mammalian nuclear phosphoprotein DEK is implicated in multiple cellular processes, including transcriptional regulation, mRNA processing, and chromatin remodeling, and is associated with a number of clinical autoimmune and neoplastic conditions. DEK, an abundant chromatin-associated protein, changes the topology of DNA in chromatin in vitro. Although first identified in a fusion with the CAN/Nup214 nucleoporin protein in a specific subtype of acute myelogenous leukemia, DEK is also an autoantigen in patients with Pauciarticular onset juvenile rheumatoid arthritis. Furthermore, the last 65 amino acids of DEK can partially reverse the mutation-prone phenotype of cells from patients with ataxia-telangiectasia. The human DEK gene maps to chromosome 6p22.3.
CITATIONS
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Wei-Jun Yang
DEK terminates diapause by activation of quiescent cells in the crustacean Artemia
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WB
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IF
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Artemia parthenogen
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Citation
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