PRODUCT CODE: ET1705-32

DDX5 Recombinant Rabbit Monoclonal Antibody [JM52-30] (ET1705-32)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.<br />
Positive control:<br />
  Lane 1: PC-12 <br />
        Lane 2: MEF <br />
  Lane 3: SH-SY5Y <br />
      Lane 4: NIH-3T3 <br />
  Lane 5: Mouse brain
  • Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.<br />
Positive control:<br />
  Lane 1: PC-12 <br />
        Lane 2: MEF <br />
  Lane 3: SH-SY5Y <br />
      Lane 4: NIH-3T3 <br />
  Lane 5: Mouse brain
  • ICC staining DDX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDX5 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DDX5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with DDX5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: MEF
Lane 3: SH-SY5Y
Lane 4: NIH-3T3
Lane 5: Mouse brain

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DDX5 Recombinant Rabbit Monoclonal Antibody [JM52-30] (ET1705-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12 cell lysate, MEF cell lysate, SH-SY5Y, NIH-3T3 cell lysate, mouse brain tissue lysate, Hela, SW480, rat brain tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM52-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69/60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

DDX5

SYNONYMS

ATP dependent RNA helicase DDX5 antibody; DDX 5 antibody; Ddx5 antibody; DDX5_HUMAN antibody; DEAD (Asp Glu Ala Asp) box helicase 5 antibody; DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody; DEAD box 5 antibody; DEAD box protein 5 antibody; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody; G17P1 antibody; HELR antibody; HLR1 antibody; HUMP68 antibody; P68 antibody; p68 RNA helicase antibody; Probable ATP dependent RNA helicase DDX5 antibody; Probable ATP-dependent RNA helicase DDX5 antibody; RNA helicase p68 antibody

SEQUENCE SIMILARITIES

Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.

POST-TRANSLATIONAL MODIFICATION

Arg-502 is dimethylated, probably to asymmetric dimethylarginine.; Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.; Polyubiquitinated, leading to proteasomal degradation.; Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.