DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33] (ET7107-41)

Western blot analysis of DDAH2 on different tissue lysates using anti-DDAH2 antibody at 1/2,000 dilution.<br />
Positive control:<br />
Lane 1: Rat heart<br />
Lane 2: Mouse heart<br />
Lane 3: Mouse lung<br />
Lane 4: Human lung

Western blot analysis of DDAH2 on different tissue lysates using anti-DDAH2 antibody at 1/2,000 dilution.
Positive control:
Lane 1: Rat heart
Lane 2: Mouse heart
Lane 3: Mouse lung
Lane 4: Human lung

Applications

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33] (ET7107-41)

Immunogen

Synthetic peptide within human ddah2 aa 1-100

Host

Rabbit

Positive Control

Rat heart tissue lysate, mouse heart tissue lysate, mouse lung tissue lysate, human lung tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB59-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

30kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:2,000-1:5,000

IP
1:10-:50

TARGET

UNIPROT #

O95865

PROTEIN NAME

DDAH2

SYNONYMS

DDAH antibody; DDAH II antibody; DDAH-2 antibody; DDAH2 antibody; DDAH2_HUMAN antibody; DDAHII antibody; Dimethylargininase 2 antibody; Dimethylargininase-2 antibody; Dimethylarginine dimethylaminohydrolase 2 antibody; Dimethylarginine dimethylaminohydrolase II antibody; G6a antibody; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 antibody; NG dimethylarginine dimethylamino hydrolase homolog antibody; NG30 antibody; OTTHUMP00000029307 antibody; OTTHUMP00000029310 antibody; OTTHUMP00000174488 antibody; OTTHUMP00000174489 antibody; Protein G6a antibody; S phase protein antibody; S-phase protein antibody

SEQUENCE SIMILARITIES

Belongs to the DDAH family.

TISSUE SPECIFICITY

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.

SUBCELLULAR LOCATION

Cytoplasm. Mitochondrion.

FUNCTION

DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.