Applications
-
WB
-
IHC-P
-
IP
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18] (ET1705-71)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human kidney tissue lysate, human liver tissue lysate, human prostate tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM53-18
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
DDAH1
SYNONYMS
DDAH antibody; DDAH I antibody; DDAH-1 antibody; DDAH1 antibody; DDAH1_HUMAN antibody; DDAHI antibody; Dimethylargininase 1 antibody; Dimethylargininase-1 antibody; Dimethylarginine dimethylaminohydrolase 1 antibody; N(G) antibody; N(G)-dimethylarginine dimethylaminohydrolase 1 antibody; NG NG dimethylarginine dimethylaminohydrolase antibody
SEQUENCE SIMILARITIES
Belongs to the DDAH family.
TISSUE SPECIFICITY
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.