DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50] (ET7110-31)

UNIPROT #

Q9UBU7

PROTEIN NAME

DBF4

SYNONYMS

Activator of S phase kinase antibody;ASK antibody;CHIF antibody;Chiffon homolog A antibody;DBF4 antibody;DBF4 homolog (S. cerevisiae) antibody;DBF4 homolog antibody;DBF4 type zinc finger containing protein 1 antibody;DBF4 zinc finger A antibody;DBF4 zinc finger antibody;DBF4, S. cerevisiae, homolog of antibody;DBF4-type zinc finger-containing protein 1 antibody;DBF4A antibody;DBF4A_HUMAN antibody;Dumbbell former 4, S. cerevisiae, homolog of antibody;OTTHUMP00000161661 antibody;Protein DBF4 homolog A antibody;ZDBF1 antibody;Zinc finger, DBF-type containing 1 antibody

TISSUE SPECIFICITY

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation increases its interaction with PSIP1.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.